Gene Library Based Approach

Testing multiple genes at once.

DoE ( Design of Experiment) approach

Machine learning on sequence-activity data

Several gene libraries are available for bottleneck identification:

Yeast pathway upregulation (MEV-ERG, chaperones, NADPH, ATP, transporters, etc.)

Dominant marker based, low copy plasmid libraries with 3-4 genes/ plasmid.

Automatized Strain Building workflows

Quick and efficient strain building workflows

DoE library screening (2 weeks)

Mini- library building- Stable integration of several multi-gene combinations (4 weeks)

Gene KO, multiplexed gene KO (2 weeks)

High copy random integration based over expression (2 weeks)

Gene up/ down regulation (promoter exchange) in 3 weeks 

Reporter gene fusions with proven reporter genes.

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