Gene Library Based Approach Testing multiple genes at once. DoE ( Design of Experiment) approach Machine learning on sequence-activity data Several gene libraries are available for bottleneck identification: Yeast pathway upregulation (MEV-ERG, chaperones, NADPH, ATP, transporters, etc.) Dominant marker based, low copy plasmid libraries with 3-4 genes/ plasmid. Automatized Strain Building workflows Quick and efficient strain building workflows DoE library screening (2 weeks) Mini- library building- Stable integration of several multi-gene combinations (4 weeks) Gene KO, multiplexed gene KO (2 weeks) High copy random integration based over expression (2 weeks) Gene up/ down regulation (promoter exchange) in 3 weeks Reporter gene fusions with proven reporter genes.
