State of The Art Analytical Instruments Instruments: Discovery applications (Proteomics, Metabolomics) UPLC + Synapt QTOF MS Sensitive Instruments for low titer targets (MS) UPLC-TQD (tandem quad) UPLC-QDa (single quad) Higher titer targets (UV detection) UPLCs HPLCs Volatile compounds GC-FID Robotized HTP sample preparation Proteomics Protein identification and quantification Peptide mapping Exact molecular weight determination Bottom- up (shotgun) proteomics Detect and quantify several hundreds of proteins simultaneously from one sample after protease digestion Applications Verification of gene knock-out Verification of gene over expression Check presence of protein "Protease Hunt"- Identify proteases Metabolomics Target Metabolomics Identification of unknown compounds using MS Untargeted Metabolomics Detect changes in the metabolite level Identify isotope labelled compounds
